EQUIPMENT

3 TRANSMISSION ELECTRON MICROSCOPES

5 SCANNING ELECTRON MICROSCOPES

DUAL BEAM FIB/SEM

LIGHT MICROSCOPES- Inverted/upright laser scanning confocal,  widefield epifluorescence with structured illumination (Apotome) and digital deconvolution, digital and film cameras, 3D reconstruction and extended focus,  standard and inverted microscopes,  DIC and phase contrast,  halogen epi-illumination,  Iatia Phase Reconstruction,  Macroscope, Polarising Microscope

SCANNING TUNNELING MICROSCOPE ("student " type)

IMAGE PROCESSING software and equipment
ULTRAMICROTOMES standard and cryo
CRYOPREPARATION EQUIPMENT large range of instruments and accessories
OTHER PREPARATION AND ANCILLARY EQUIPMENT

 

TRANSMISSION ELECTRON MICROSCOPES

Hitachi H7100FA (125kV TEM, 1995) with digital camera

Used for general and cold stage work not requiring high tilt.
Side-entry (limited)tilt stage. Fine probe capability (20-100nm) 0.144 nm lattice resolution. Double diffusion pump vacuum system. Electronic image rotation. Standard single-tilt, double-tilt and high resolution diffraction/reflection specimen holders. W or LaB6 cathode. 22 plate large format camera. Specimen movement trace. Point-to-point measurement. PC Interface. LN2 Cold Stage (Oxford Instruments). Gatan 622SC CCD TV camera with image intensifier. VCR. SIS Megaview III Widefield CCD camera (1300 x 1024 pixel, 12 bit).

Philips EM430 with digital camera (300kV TEM/STEM, bought 1985, transferred to ANUEMU and upgraded 1998)

Used for materials science or biological work requiring higher resolution, high tilt/high kV or X-ray analysis
Side-entry high-tilt stage. Diffusion/ion pump system. LaB6 or tungsten. Wide range of specimen holders including double tilt, hot and cold stages. Low dose unit. Ultra-thin window Oxford ISIS EDXA with mapping. Direct and annular STEM, digitised through ISIS. TVIPS 1024 x 1024 24µm 12 bit CCD camera and image processing system.  36 plate large format sheet film camera.

Philips CM300 with digital camera (300kV TEM. 1999)

Sited in RSES. Gatan 1024 x 1024 CCD camera, EDAX EDXA. Contact Dr John Fitz Gerald (John.Fitzgerald@anu.edu.au) or phone (02) 6125 4176.

SCANNING ELECTRON MICROSCOPES

Cambridge S360 (SEM, 1987)

Used for general purpose SE and BSE imaging, cryoSEM and X-ray analysis.
LaB6, Tracor Northern EDXA detector (Be window, 147eV) with Moran EDXA processing system, 4-segment backscatter detector, Oxford CT1500B Cryotrans cold stage/coating unit , point-to point measurement intrinsic to microscope. Turbomolecular and sputter ion pumps,. 70mm ("220") roll film, videographic printer, ImageSlave 1024 x 768 slow-scan image acquisition.

JEOL JSM6400 (SEM, 1990)

Oxford ISIS EDXA with ATW window (sensitive down to boron), quantitative and qualitative elemental mapping, CAMEO " chemical imaging" system. Robinson backscatter detector, forescatter detector, transmitted electron detector, LaB6 or tungsten filament. Diffusion and sputter ion pumps, 70mm roll film, videographic printer, video output, ImageSlave 1024 x 768 slow-scan image acquisition to networked PC.

Hitachi S2250-N (Conventional and low-vacuum SEM, 1992)

Sited in RSES. Used mainly for cathodoluminescence imaging, May also be available for uncoated/unfixed specimens, tensile stages etc. (Contact access through ANUEMU).

Tungsten filament, diffusion pump. Gas pressure in VP mode up to 2 torr air, allows viewing uncoated specimens. Secondary (E-T) and Robinson backscatter/SE detectors.  Framestore, video output.   (Not currently in use- Fullam Peltier-cooled stage, manipulators, tensile/compression stages, absorbed electron detector, beam blanking unit). ImageSlave slow-scan image acquisition (1024 x 853) .  Cathodoluminescence -  panchromatic imaging (off-axis mirror and PMT) installed  and spectrographic (fibre optic and Ocean Optics CCD spectrometer) available.

Hitachi S4500 (Cold Field Emission SEM, 1996)

High resolution, low kV, "semi in-lens" design. 0.5 to 30kV. Diffusion/ion pump cascade system. "Upper and lower" secondary electron detectors, Robinson Mk 6 backscatter detector, Oxford high resolution cold stage with magnetron sputter coater, ImageSlave slow-scan image acquisition (1024 x 768). 70mm roll film camera, video output etc.

Hitachi 4300 SE/N (Schottky Field Emission SEM, 2006)

Analytical below-lens design VP to 1000Pa. SE, YAG BSE and gaseous SE detector (biassed absorbed current type). Fitted with Oxford Instruments cold stage and INCA Energy 350 EDXA system. HKL EBSP, Robinson CL, 2-axis motorised stage. IR Camera.

Zeiss UltraPlus analytical FESEM (2008)

1.8nm resolution at 1 kV. Angular sensitive Backsactter (AsB), Energy sensitive Backscatter (EsB), Upper an lower SE detectors, gas injection needle for charge compensation. Fitted with Oxford Instruments cold stage and INCA Energy 450 EDXA system plus Oxford HKL EBSP,  5-axis motorised stage. IR Chamber scope.

LIGHT MICROSCOPES

Nikon Optiphot epi-fluoro,  (phase and epi-fluoro objective turrets) with SPOT Insight 1600 x 1200 mono 12 bit camera, Iatia QPm Quantitative Phase System. Sony 3-chip video camera

Wild M400 photomacroscope with SPOT FLEX CCD camera 14bit  50Mb colour CCD camera. Upgraded with LEICA illumination base.

Nikon inverted microscope

Leitz Polarising microscope

2 Nikon Labophots, one with phase contrast, halogen trans and epi-illumination. 5 to 100x objectives
Small "dissecting" stereo-microscopes
Wild stereo-microscope with observer tube

Videoflex Macro video camera
Video output from  microscopes and Videoflex,, linked to videorecorder, image analysis PC, videoprinter.
Spectrometer adapter available for light microscopes.

 

IMAGE PROCESSING

Image and data analysis, Digitising/measurement systems, 3-D reconstruction, Image Processing and Printing.

The"Fishbowl",  Rm 035, contains light microscopes and , PCs with image handling software and a variety of input and output devices including a high resoluton Epson flatbed scanner with transparency option,  and a large digitising tablet.  It is also used for processing digital images taken directly from the electron microscopes. Equipment in this area is continually being upgraded, so check to see what is available from time to time.

SOFTWARE

For PCs : (4 stations)
Various options for image presentation, 2D analysis, deconvolution and 3D reconstruction including AnalySIS, Image Pro, ImageJ, IrfanView,  ImageJ, Offline Leica confocal software, Zeiss Axiovision, .Adobe Photoshop,  Sigma-Scan Pro,  Surfdriver (3-D recon).  Lucis image processing,

OUTPUT DEVICES

Network access to 1200 dpi. postscript laser printers. In general colour and photoquality printing is not supported by the EMU.   Most PCs are networked, and have CD-RW, and DVD drives.   BYO USB drives are recommended. 

ULTRAMICROTOMES

5 Reichert Ultracuts, two with cryo-sectioning attachments.  Staining area

 
CRYO- EQUIPMENT

LN2 Cold stages  on 2 SEMs and 3 TEMS

High Pressure Freezer
Leica EM-PACT. Pressure controllable to 2300 bar. Specimen holders currently 350 micron x 17mm tubes, or c. 2mm dia x 200 micron deep larger flat holders.

Leica Freeze-substitution ..programmable

Cryopliers

Propane Jet Freezer  Bal-Tec JFD 030 Twin jet freezer

"Controlled Environment Vitrification System"  For fast plunging into liquid ethane or propane, particularly for TEM grids

Freeze-fracture  Balzers 301 with turbomolecular pump, electron beam evaporation guns, double replica device

Freeze-drying  Routine - Dynavac with Peltier-cooled stage, rotary pump

Freeze drying  Small sample/High-resolution- Polaron E7480, LN2 cooled with sputter and evaporative coating units, diffusion pump.

OTHER FACILITIES

Sputter Coater (high res) Pt or AU, Bio-Rad


Sputter Coater (Au/Pd): Emitech

Evaporative Coater Dynavac CS300, diffusion pumped

Glow Discharge Unit: Emitech. Reversible polarity, gas

Critical point dryer Balzers

Ocean Optics portable optic fibre spectrometer

Pelco BioWave Microwave Fixation Oven. 80w to 750w, vacuum chamber,  temperature restriction, programmable

Biological Specimen Processing  A number of fixatives, buffers, resins etc are available as stock. A dissecting microscope is available for use within the fumehood.  Before using this area, see staff for explanation of features and safety procedures.

Polishing / Grinding area. Setting up- wheel  with abrasive paper, cloths and paste

Fumehoods, ultrasonic bath, bench microfuge, ovens, forceps sharpening kit, stereo viewers, balances, shaker, VCRs.